Sequencing of Mx gene expression in Labeo rohita

Mx genes are inducible by type I interferon and involved in antiviral defenses in vertebrates. In the present investigation, the protocol for obtaining Mx gene expression was standardized by Poly I:C injection in Labeo rohita. A 786 bp cDNA was obtained on day 3 after poly I:C injection. PCR amplification of this cDNA using Mx specific primers also yielded 786 bp product. However, PBS injected fishes did not show any Mx expression.

Sharma, ParvatiMyself Dr. Parvati Sharma, Assistant Professor, Department of Zoology, Chaudhary Bansi lal University, Bhiwani. I have got two gold medals in academic.Presently I am working on probiotics which are beneficially bacteria present in bovine and non bovine products and help in nutrition of gastrointestinal tract of the body through various mechanisms.